Publications

Development and validation of a universal blood donor genotyping platform: a multinational prospective study

In our first publication we report on the development and initial evaluation of the Applied Biosystems™ Axiom™ UK Biobank_v2 (UKBB_v2) DNA genotyping array for antigen genotyping of donors and patients.

In this study we:

1. Designed a DNA genotyping array capable of typing almost all known Red Cell (HEA), Platelet (HPA), and Leukocyte (HLA) antigens
2. Evaluated our array using DNA samples from 7,984 blood donors from England and the Netherlands
3. Compared inferred antigen genotyping results to those on donor record

Our results showed:

• 99.9% concordance in 101,676 comparisons between our antigen genotypes and previous typing results
• Genotyping increased the total number of antigen typing results available from 110,980 to >1,200,000
• Dense typing results greatly simplifies the challenge of finding compatible blood for complex patients

You can read the full publication here: Gleadall, et al., Development and validation of a universal blood donor genotyping platform: a multinational prospective study. Blood Advances, 2020.

Associated Publications

Lane, et al., GYPB gene deletions associated with the U− phenotype in those of African ancestry. Transfusion, 2020.

Turro, et al., Whole-genome sequencing of patients with rare diseases in a national health system. Nature, 2020.

Lane, et al., Automated typing of red blood cell and platelet antigens: a whole-genome sequencing study. Lancet Haematology, 2018.